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GenScript corporation wheat u6 promoters and wheat grna scaffolds
Wheat U6 Promoters And Wheat Grna Scaffolds, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wheat+u6+promoters+and+wheat+grna+scaffolds/wheat+u6+promoters+and+wheat+grna+scaffolds/us10988775-388-5-11
Average 90 stars, based on 1 article reviews
wheat u6 promoters and wheat grna scaffolds - by Bioz Stars, 2026-09
90/100 stars

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Synthesized:

Article Title: Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew.
Article Snippet: Sequence-specific nucleases have been applied to engineer targeted modifications in polyploid genomes1, but simultaneous modification of multiple homoeoalleles has not been reported.. Here we use transcription activator–like effector nuclease (TALEN)2,3 and clustered, regularly interspaced, short palindromic repeats (CRISPR)-Cas9 (refs. 4,5) technologies in hexaploid bread wheat to introduce targeted mutations in the three homoeoalleles that encode MILDEWRESISTANCE LOCUS (MLO) proteins6.. Genetic redundancy has prevented evaluation of whether mutation of all three MLO alleles in bread wheat might confer resistance to powdery mildew, a trait not found in natural populations7.

Article Title: Wheat plants resistant to powdery mildew
Article Snippet: Full-length Cas9 (plant codon-optimized) products were digested with BamHI and MfeI and inserted into plasmid pJIT163-Ubi between the BamHI and MfeI sites to yield the expression vector pJIT163-Ubi-Cas9. .. The wheat U6 promoters and wheat gRNA scaffolds were synthesized by GenScript and cloned into pEASY-blunt vector (TransGen Biotech). ..

Clone Assay:

Article Title: Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew.
Article Snippet: Sequence-specific nucleases have been applied to engineer targeted modifications in polyploid genomes1, but simultaneous modification of multiple homoeoalleles has not been reported.. Here we use transcription activator–like effector nuclease (TALEN)2,3 and clustered, regularly interspaced, short palindromic repeats (CRISPR)-Cas9 (refs. 4,5) technologies in hexaploid bread wheat to introduce targeted mutations in the three homoeoalleles that encode MILDEWRESISTANCE LOCUS (MLO) proteins6.. Genetic redundancy has prevented evaluation of whether mutation of all three MLO alleles in bread wheat might confer resistance to powdery mildew, a trait not found in natural populations7.

Article Title: Wheat plants resistant to powdery mildew
Article Snippet: Full-length Cas9 (plant codon-optimized) products were digested with BamHI and MfeI and inserted into plasmid pJIT163-Ubi between the BamHI and MfeI sites to yield the expression vector pJIT163-Ubi-Cas9. .. The wheat U6 promoters and wheat gRNA scaffolds were synthesized by GenScript and cloned into pEASY-blunt vector (TransGen Biotech). ..

Plasmid Preparation:

Article Title: Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew.
Article Snippet: Sequence-specific nucleases have been applied to engineer targeted modifications in polyploid genomes1, but simultaneous modification of multiple homoeoalleles has not been reported.. Here we use transcription activator–like effector nuclease (TALEN)2,3 and clustered, regularly interspaced, short palindromic repeats (CRISPR)-Cas9 (refs. 4,5) technologies in hexaploid bread wheat to introduce targeted mutations in the three homoeoalleles that encode MILDEWRESISTANCE LOCUS (MLO) proteins6.. Genetic redundancy has prevented evaluation of whether mutation of all three MLO alleles in bread wheat might confer resistance to powdery mildew, a trait not found in natural populations7.

Article Title: Wheat plants resistant to powdery mildew
Article Snippet: Full-length Cas9 (plant codon-optimized) products were digested with BamHI and MfeI and inserted into plasmid pJIT163-Ubi between the BamHI and MfeI sites to yield the expression vector pJIT163-Ubi-Cas9. .. The wheat U6 promoters and wheat gRNA scaffolds were synthesized by GenScript and cloned into pEASY-blunt vector (TransGen Biotech). ..



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GenScript corporation wheat u6 promoters and wheat grna scaffolds
Wheat U6 Promoters And Wheat Grna Scaffolds, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wheat+u6+promoters+and+wheat+grna+scaffolds/wheat+u6+promoters+and+wheat+grna+scaffolds/us10988775-388-5-11
Average 90 stars, based on 1 article reviews
wheat u6 promoters and wheat grna scaffolds - by Bioz Stars, 2026-09
90/100 stars
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